2 Funded Postdoctoral positions on engineered EVs for RNA delive
Karolinska Institutet, Institutionen för laboratoriemedicin / Biologjobb / Huddinge
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hela Sverige The Department of Laboratory Medicine consists of 9 divisions and is active in many areas of Laboratory Medicine. The department is involved in advanced basic scientific, clinical and epidemiological research. The department is one of the largest departments of education at Karolinska Institutet.
The division
The Department of Laboratory of Medicine, located in Huddinge Campus, is one of the Karolinska Institutet's largest departments with 9 research divisions including the Clinical Research Centre (CRC), where the project will be carried out.
350 people are employed at the department, half of which are PhD-students. The Centre provides the facilities needed for cell- and molecular biology research and the possibility to perform research in vivo.
Our research efforts in EL Andaloussi 's lab focus on one of the greatest challenges facing modern therapeutics; delivery. Despite the unprecedented knowledge of diseases and their mechanisms due to advances in biomedical sciences; many promising therapeutic approaches are still clinically unavailable. This is simply because there is no efficient means of delivering these therapeutics to the right organ with the right dose. Such therapeutics include proteins for replacement therapy or antibodies for anti-inflammatory treatment as well as nucleic acids for gene therapy of diseases including muscular dystrophies and neurodegenerative disorders. We develop innovative drug delivery technologies that are able to carry protein and/or gene therapies to the target tissues safely and efficiently. We have developed a promising technology based on reprogramming exosomes, which are vesicles that are naturally used by cells to communicate, to carry therapeutic proteins and nucleic acids. We engineer exosomes with enhanced delivery and targeting capacities and screen our wide array of exosome designs in disease-relevant cellular and animal models. We are also developing methods for large-scale production and purification of such exosome therapeutics. Additionally, our lab develops methods for gene therapy based on modified cell-penetrating peptides that are efficient carriers of nucleic acids and proteins across biological barriers. We test our different technologies in models of inflammatory diseases as well as neurodegenerative and neuromuscular disorders. Our main aim is to one day unlock the potential of protein and gene therapy of such diseases by the development of novel and efficient delivery technologies.
Duties
The post doc project will mostly revolve around the development of bioengineered extracellular vesicles for delivery of RNA-based therapeutics.
Most of the research will be conducted utilizing cell lines and animal models. The first part of the work will involve engineering of therapeutically active exosomes with different RNA species. This is achieved by cloning various genetic constructs that are introduced into producer cells and validated for expression in purified exosomes. The second part will involve testing these exosomes in normal and tumor-bearing mice.
The applicants should have a solid background in basic biology or biotechnology. Practical lab experience is essential, especially in the following areas: expression and purification of extracellular vesicles, fluorescent/confocal microscopy (ICC and IHC), protein analysis (Western blot, ELISA and IP), and cell culture work. It is crucial with previous experience from extensive work with mice. For one of the positions it is crucial to hold a FELASA certificate. For the other position it is highly merited to hold a FELASA certificate.
Previous experience from molecular cloning is a merit. In addition, excellent proficiency in both spoken and written English is highly desired. We are looking for an energetic and passionate person who fits in, and actively contributes to our team.
Entry requirements
A person is eligible for a position as Postdoctoral Researcher if he or she has obtained a PhD no more than seven years before the last date of employment as postdoc. The term can be extended under special circumstances.
Application process
An employment application must contain the following documents in English or Swedish:
- A complete curriculum vitae, including date of the thesis defence, title of the thesis, previous academic positions, academic title, current position, academic distinctions, and committee work
- A complete list of publications
- A summary of current work (no more than one page)
- Verifications for crediting of illness, military service, work for labour unions or student organisations, parental leave or similar circumstances
The application is to be submitted through the MyNetwork recruitment system.
Karolinska Institutet is one of the world's leading medical universities. Its vision is to significantly contribute to the improvement of human health. Karolinska Institutet accounts for the single largest share of all academic medical research conducted in Sweden and offers the country's broadest range of education in medicine and health sciences. The Nobel Assembly at Karolinska Institutet selects the Nobel laureates in Physiology or Medicine.
Pursuant to the regulations of the Swedish National Archives, applications are kept on file for two years after the appointment has gained legal force. The regulations do not apply to attachments that have been printed or otherwise published.
Karolinska Institutet strives to provide a workplace that has approximately the same number of women and men, is free of discrimination and offers equal opportunity to everyone.
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Varaktighet, arbetstid
100% Visstidsanställning
Publiceringsdatum2017-10-16Ersättningfast
Så ansöker duSista dag att ansöka är 2017-10-29
Ange följande referens när du ansöker: 2-4572/2017
Klicka på denna länk för att göra din ansökanKontaktSamir El-Andaloussi
Samir.El-Andaloussi@ki.seFöretagKarolinska Institutet, Institutionen för laboratoriemedicin
AdressKarolinska Institutet, Institutionen för laboratoriemedicin
Administrativa Kansliet, 8100, Alfred Nobels Allé
14183 Stockholm
KontorsadressAdministrativa Kansliet, 8100, Alfred Nobels Allé
Jobbnummer 3751021
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