PPhD student in Immuntechnology
Lunds Universitet / Biologjobb / Lund
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hela Sverige PhD student in Immunotechnology
Type of employment: Limit of tenure, June 30, 2018
Extent: 100 %
Location: Department of Immunotechnology, Lund
First day of employment: July 1, 2014
Official Records Number: LTHPA 2014/397
This advert is to find a candidate to fill the position of early stage researcher ESR7 in the Marie Curie Initial Training Network (MC-ITN) called ReBirth. The reBIRTH consortium focuses on molecular mechanisms of age-associated pathologies including neurodegeneration and depression. The nine international groups and companies work together to identify stress-regulated molecules provoking neuronal death and hindering neurogenesis, and monitor the consequences of these processes in human brain. The concept is that intelligent targeting of stress signaling will act synergistically to improve brain function in depressive disorders and ageing. The consortium is described in more detail at:
http://www.itn-rebirth.euThe candidate will work on the identification of novel markers of adult neurogenesis. This will be done in the laboratory of Peter James in Lund (Sweden) with stays in the groups of Eleanor Coffey in Turku (Finland) and in the phage antibody development company Immunovia in Lund (Sweden).
Job assignments
(i) The first aim is to first identify membrane protein markers of neurogenesis in order to be able to raise antibodies to allow the identification of neurogenic cells. Neurogenesis discontinues in ageing brain and subtractive proteomics will be used to identify markers by comparing neurogenic-incompetent cells from the dentate gyrus in aged mouse brain with neurogenic competent cells from young brain. Laser dissection and FACS sorting will be used to enrich for these cells after gross dissection and tissue disruption. HPLC-MS/MS analysis of plasma membrane enriched preparations will be carried out using low-specificity protease digests methods developed in the James laboratory and by glycoprotein affinity capture.
(ii) Once partners have developed antibodies to allow a more accurate FACS sorting using the markers found in (i) the student will analyse the JNK signalling pathways in these cells isolated from mouse models with JNK gene knockouts and comparing with the wild type phosphorylation patterns and follow how these change with ageing. In parallel, the effect of specific JNK inhibitors will be studied as well as the response to changes in endocrine signalling.
The candidate will learn advanced mass spectrometry using label-free and isotopic labelling techniques as well as glyco-affinity capture and be trained in bioinformatics for the analysis of pathways and protein function.
Eligibility/Entry Requirements
Students with basic eligibility for third-cycle studies are those who- have completed a second-cycle degree- have completed courses of at least 240 credits, of which at least 60 credits are from second-cycle courses, or- have acquired largely equivalent knowledge in some other way, in Sweden or abroad.Special eligibility requirements comprise knowledge from first-cycle studies or the equivalent, but can also refer to special professional experience. Additionally, sufficient knowledge of the subject area is required for third-cycle studies.Students meeting special eligibility requirements are those who have- at least 60 higher education credits within the same subject area as the third-cycle studies, of which in-depth work of at least 30 higher education credits of relevance to the subject area- a Swedish medical degree or Swedish certification as a doctor.
The position is connected to the Marie Curie Initial Training Network r'BIRTH. To be eligible for the position the holder must not have resided or carried out their main activity (work, studies etc.) in Sweden for more than 12 months during the last 3 years.
Basis of Assessment
The employment of doctoral students is regulated in the Swedish Code of Statues 1998: 80. Only those who are or have been admitted to PhD-studies may be appointed to doctoral studentships. When an appointment to a doctoral studentship is made, the ability of the student to benefit from PhD-studies shall primarily be taken into account. In addition to devoting themselves to their studies, those appointed to doctoral studentships may be required to work with educational tasks, research and administration, in accordance with specific regulations in the ordinance.
The applicant must have experience working with animals, preferably in neurophysiology. Knowledge of proteomics techniques, both protein and peptide separation will be of great value. Experience with protein phosphorylation is an advantage. The ITN requires that the candidate be flexible and keen to work in several environments that cover the specialist areas that make up the work package.
The applicant should have experience working with cell culture. Knowledge of proteomics techniques, including both protein and peptide separation as well as protein purification will be of great value. The ITN requires that the candidate be flexible and keen to work in several environments that cover the specialist areas that make up the work package.
The candidate will learn advanced mass spectrometry using label-free and isotopic labelling techniques as well as crosslinking interaction capture and be trained in bioinformatics for the analysis of pathways and protein function.
Publiceringsdatum2014-05-15Arbetstider och omfattningTillsvidare
Heltid
Please apply online. Complementary documents, such as publications/books should be sent to: Registry office, Lund University, P.O. Box 117, SE-221 00 LUND, SWEDEN. The official record number should be clearly stated when sending complementary documents
Tillträde140701
ErsättningFast lön
Salary is determined on an individual basis, within certain guidelines for this type of position. Please state requested salary in your application.
Så ansöker duSista dag att ansöka är 2014-06-06
Lund University promotes an equal opportunities code. We encourage both men and women to apply for this position
Applications will be destroyed or returned (upon request) two years after the decision of employment has become final. Applications from the employed and from those who appeal the decision will not be returned.
Ange följande referens när du ansöker: LTHPA 2014/397
KontaktPeter James Professor 046-2221496
Peter.James@immun.lth.seFöretagLunds Universitet
AdressLunds Universitet
Box 117
22100 LUND
Övriga kontaktuppgifterTelefonnummer: 046-2220000
Jobbnummer 1876497
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